Trypsin Trypsin (EC 3.4.21.4) — High-Purity Protease for Controlled Cleavage
Consistent high-activity Trypsin for reliable proteolysis across cell culture, biomanufacturing, and analytical workflows. Activity ≥2,500 USP units/mg, optimal pH 7.0–9.0 at 37°C. White to off-white lyophilized powder with a shelf life of 36 months (-20°C) or 12 months (2–8°C). Pack sizes 1 g / 5 g / 100 g / 1 kg. MOQ 1 g (research) / 100 g (bulk).
Quality USP/NF & cGMP
Product Specifications
01 Enzyme Name
Trypsin (EC 3.4.21.4)
02 Activity
USP: 2,500+ USP units/mg
03 pH Range
7.0 – 9.0
04 Temperature
37°C
05 Appearance
White to off-white lyophilized powder
06 Source
Bovine/Porcine pancreas, Recombinant (corn)
07 Shelf Life
36 months (-20°C), 12 months (2-8°C)
08 Packaging
1 g / 5 g / 100 g / 1 kg
09 MOQ
1 g (research) / 100 g (bulk)
10 Certifications
USP/NF, cGMP, Animal-Free available, Irradiated available
Applications
Trypsin is a serine protease used for precise peptide-bond cleavage in validated industrial and laboratory workflows. Commonly applied in cell dissociation, vaccine manufacturing, proteomics digestion, insulin processing, and pharmaceutical enzymatic debridement within defined pH and temperature ranges.
Cell Culture & Passaging
Standard cell dissociation enzyme for adherent cell cultures—detaches cells from culture vessels for passaging.
Learn more →Vaccine Production
Cell harvesting in vaccine manufacturing—trypsinization of Vero, MDCK, and HEK293 cells at production scale.
Proteomics & Mass Spec
In-gel and in-solution protein digestion for bottom-up proteomics—the gold standard for LC-MS/MS sample prep.
Insulin Production
Proteolytic processing of proinsulin to active insulin—critical step in recombinant insulin manufacturing.
Wound Debridement
Pharmaceutical-grade trypsin for enzymatic wound debridement—removes necrotic tissue in chronic wound care.
Recombinant (Animal-Free)
TrypLE and corn-derived recombinant trypsin—animal-origin-free alternative for GMP cell therapy manufacturing.
Learn About Trypsin
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Frequently Asked Questions
Q1 What is Trypsin (EC 3.4.21.4) primarily used for?
Trypsin is commonly used for 🧫 cell dissociation/passaging, 💉 vaccine cell harvesting, 🧬 proteomics digestion for LC-MS/MS, 💊 proinsulin processing in insulin manufacturing, and 🩹 enzymatic wound debridement.
Q2 What are the recommended pH and temperature for Trypsin activity?
For optimal performance, use Trypsin at pH 7.0–9.0 and 37°C, matching typical cell culture and proteolysis conditions.
Q3 How should I interpret “USP: 2,500+ USP units/mg” on the label?
This reflects enzyme activity per milligram based on USP testing; higher activity can enable faster cleavage at equivalent dosage, pending application-specific validation.
Q4 Do you offer animal-free Trypsin options?
Yes. Animal-free options are available, including recombinant (corn) trypsin and related alternatives suitable for GMP cell therapy manufacturing.
Q5 What storage conditions maximize shelf life?
Shelf life is 36 months at -20°C and 12 months at 2–8°C. Keep the lyophilized powder sealed and follow COA/TDS storage guidance provided with your shipment.
Certifications & Standards
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