Trypsin Trypsin (EC 3.4.21.4) — Serine Protease for Protein Cleavage
High-activity Trypsin for consistent proteolytic performance in cell culture, bioprocessing, and analytical workflows. Works best within recommended pH and temperature ranges. Activity 2,500+ USP units/mg. Optimal pH 7.0–9.0 and 37°C. White to off-white lyophilized powder. Shelf life up to 36 months at -20°C. Packaging 1 g / 5 g / 100 g / 1 kg. MOQ 1 g (research) / 100 g (bulk).
Quality USP/NF & cGMP
Product Specifications
01 Enzyme Name
Trypsin (EC 3.4.21.4)
02 Activity
USP: 2,500+ USP units/mg
03 pH Range
7.0 – 9.0
04 Temperature
37°C
05 Appearance
White to off-white lyophilized powder
06 Source
Bovine/Porcine pancreas, Recombinant (corn)
07 Shelf Life
36 months (-20°C), 12 months (2-8°C)
08 Packaging
1 g / 5 g / 100 g / 1 kg
09 MOQ
1 g (research) / 100 g (bulk)
10 Certifications
USP/NF, cGMP, Animal-Free available, Irradiated available
Applications
Trypsin is a serine protease used to cleave peptide bonds and enable controlled protein processing. It is widely applied in cell dissociation, biomanufacturing, proteomics digestion, and pharmaceutical enzymatic debridement when operated within validated conditions.
Cell Culture & Passaging
Standard cell dissociation enzyme for adherent cell cultures—detaches cells from culture vessels for passaging.
Vaccine Production
Cell harvesting in vaccine manufacturing—trypsinization of Vero, MDCK, and HEK293 cells at production scale.
Proteomics & Mass Spec
In-gel and in-solution protein digestion for bottom-up proteomics—the gold standard for LC-MS/MS sample prep.
Insulin Production
Proteolytic processing of proinsulin to active insulin—critical step in recombinant insulin manufacturing.
Wound Debridement
Pharmaceutical-grade trypsin for enzymatic wound debridement—removes necrotic tissue in chronic wound care.
Recombinant (Animal-Free)
TrypLE and corn-derived recombinant trypsin—animal-origin-free alternative for GMP cell therapy manufacturing.
Why Source From Us
Frequently Asked Questions
Q1 What is Trypsin (EC 3.4.21.4) primarily used for?
Trypsin is commonly used for 🧫 cell dissociation/passaging, 💉 vaccine cell harvesting, 🧬 proteomics digestion for LC-MS/MS, 💊 proinsulin processing in insulin manufacturing, and 🩹 enzymatic wound debridement.
Q2 What are the recommended pH and temperature for Trypsin activity?
For best performance, use Trypsin at pH 7.0–9.0 and 37°C, matching typical cell culture and proteolysis conditions.
Q3 How should I interpret “USP: 2,500+ USP units/mg” on the label?
This indicates the measured enzyme activity per gram based on USP testing. Higher activity generally supports faster cleavage at the same mass; final dosing should be confirmed in your specific application.
Q4 Do you offer animal-free Trypsin options?
Yes. Animal-free options are available, including recombinant (corn) trypsin and related alternatives suitable for GMP cell therapy manufacturing.
Q5 What storage conditions maximize shelf life?
Shelf life is 36 months at -20°C and 12 months at 2–8°C. Keep the lyophilized powder sealed and follow the COA/TDS storage guidance provided with your order.
Certifications & Standards
Get a Free Sample & Quote
Tell us your application and target volume. We’ll send a free sample, COA, and a competitive quote within 24 hours.
Years experience
Countries shipped